32P-postlabeling high-performance liquid chromatography (32P-HPLC) adapted for analysis of 8-hydroxy-2'-deoxyguanosine
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چکیده
منابع مشابه
Detection of multiple polycyclic aromatic hydrocarbon-DNA adducts by a high-performance liquid chromatography-32P-postlabeling method.
A 32P-postlabeling procedure for identifying and quantifying hydrophobic DNA adducts was developed (by modifying the method of Randerath and co-workers) in which labeled adducts are separated by high-performance liquid chromatography (HPLC) and quantified by liquid scintillation counting. This method was first developed for fluoranthene-DNA adducts, and methods for optimal detection and quantif...
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We have previously described a 32P assay for the detection and quantitation of aromatic carcinogen:DNA adducts (R. C. Gupta et al., Carcinogenesis (Lond.), 3: 1081-1092, 1982). The method entails enzymatic digestion of DNA to deoxynucleoside 3'-monophosphates which are then converted to deoxynucleoside 3',5'-[5'-32P]diphosphates by T4 polynucleotide kinase-catalyzed 32P transfer from adenosine ...
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Many antitumor agents, including the mustards, form N-7 deoxyguanosine adducts in DNA that are difficult to quantitate by the 32P-postlabeling procedure because of their instability. We have developed a method that is successful for the analysis of such adducts using, as a prototype mustard, 14C-labeled bis(2-chloroethyl)sulfide. This agent forms the unstable product 7-hydroxyethylthioethyldeox...
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The "P-postlabeling procedure, developed originally by Randerath and coworkers, has been modified for the detection and analytical quantitation of O'-alkyl-2'-deoxyguanosine residues in DNA. Chromato graphie techniques were developed to resolve individually the normal deoxyribonucleotide-3'-monophosphates and the O'-alkyldeoxyguanosine-3'-monophosphates by high-pressure liquid chromatography. S...
متن کاملHigh-performance liquid chromatographic analysis of 32P-postlabeled DNA-aromatic carcinogen adducts.
The technique of 32P postlabeling of DNA-carcinogen adducts is a useful and extremely sensitive method of detecting and quantitating DNA damage by carcinogens. We have adapted the 32P method to analysis by high-pressure liquid chromatography, making the procedure more rapid and convenient than when thin-layer chromatography is used. Following DNA isolation and hydrolysis, nucleotide-carcinogen ...
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ژورنال
عنوان ژورنال: Carcinogenesis
سال: 1999
ISSN: 1460-2180
DOI: 10.1093/carcin/20.7.1241